SV-AUC
(Sedimentation Velocity Analytical Ultracentrifugation)
Unlock profound insights into macromolecular behavior with our Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC) service. Our expert team utilizes state-of-the-art technology to offer comprehensive analyses, guiding your research and development efforts with unparalleled accuracy and depth. Explore the dynamic world of SV-AUC and elevate your understanding of complex molecular systems today.
About SV-AUC
Sedimentation velocity AUC is an absolute technique that measures the time required for a biological molecule to be settle in an AUC cell under centrifugal force. Over time, the boundary layer moves towards the bottom of the cell. This time dependent concentration profile is detected by absorbance optics during the SV-AUC run. The distance that the boundary layer travels between time points depends on the size, shape and mass of the molecules being sedimented. This dynamic profile reveals valuable insights into the distribution of differently sized molecules in solution (i.e. aggregates).
Why SV-AUC Matters
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AUC measures the fundamental property of mass and gravitation. It has broad applicability and can be used to analyze a wide range of solvents.
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AUC can report the absolutely molecular mass of monomer and high molecule species of wide range of biological products (1KDa-10GDa).
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There are no complications due to interactions with matrices or surfaces during AUC measurement, therefore AUC measure the homogeneity of biological product in their native state in the exact or nearly exact formulation solution conditions.
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AUC measurement is nondestructive, samples may be recovered for further tests following AUC
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AUC can be operated over a range of speeds (from 3 K to 60 K rpm). Hence, it can characterize the molecules from a few hundred daltons to almost a micron in size
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Sedimentation equilibrium is very useful to measure the reversible self-association of biological molecules in its formulation buffer or biological systems
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Orthogonal method that often required by agency to validate SEC.
Applications of SV-AUC
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Viruses characterization for vaccine and gene therapy biopharmaceutical industry:
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Characterization of virus by determine the percentage of packed, partially packed, empty viruses and virus’ aggregates
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Comparability of lot-to-lot variability of virus
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Assessing process development by providing the percentage of packed, partial packed, empty viruses virus’ aggregates for different process
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Product characterization, comparability and similarity assessments:
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Product characterization and stability test
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Comparability of lot-to-lot variability of biopharmaceutical molecules due to manufacture changes (e.g. raw material, site changes, process changes etc.)
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Generic products and originator products similarity assessment
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Orthogonal method for SEC methods
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Orthogonal method for determine the mole mass of a small peptide molecule
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Assess molecule-molecule interaction including self association, protein-liagant, protein-DAN and other interactions and equilibrium between monomer/dimer
Contact us today to harness the power of SV-AUC and enhance your biotechnology research. (Inquire more regarding SE-AUC).
Examples:
Virus Comparability by SV-AUC
The three lots of viral samples are comparable and their completely assembled virus (holovirus) monomer sedimentation coefficients are equivalent within the expected measurement variability.
Lot 1 and 2 viral samples have similar monomer sedimentation coefficients and their completely assembled virus (holovirus) are equivalent within the expected measurement variability. However, lot 1 is much less homogenous than virus 2; there are more empty and partial filled capsids.
Virus Characterization and Process Evaluation by SV-AUC
Process 1 and 2 viral samples have similar monomer sedimentation coefficients and their completely assembled virus (holovirus) are equivalent within the expected measurement variability. However, the ratio of empty capsid and fully packed holovirus is different.
Lot 1, 2 and 3 viral samples have similar monomer sedimentation coefficients and their completely assembled virus (holovirus) are equivalent. However, lot 3 has most fully packed holovirus and lot 1 has most partial packed virus.
Protein 1
Product Characterization by SV-AUC
Protein 1 is highly homogeneous.
Protein 2
Protein 2 is fairly homogeneous, showing monomer and high molecular weight species.
Comparability Assessment by SV-AUC
The monomer sedimentation coefficients for six lots of mAb samples are equivalent within the expected measurement variability.
The monomer sedimentation coefficients for three lots of protein samples are equivalent within the expected measurement variability.