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Dr. Tiansheng Li, the founder of HTL Biosolutions, has left an indelible mark on the landscape of biotechnology. His unwavering commitment to excellence, innovative thinking, and passion for Raman spectroscopy and formulation development defined HTL's ethos and propelled it to new heights.

Dr. Li's vision was not merely about building a successful enterprise but about being a catalyst for healing and progress in the field of biopharmaceuticals by empowering other companies in advancing their innovative biopharmaceuticals and contributing to transformative healthcare solutions. His legacy lives on in the services, technologies, and culture at HTL Biosolutions. Dr. Li's pioneering spirit continues to inspire the dedicated team he assembled, fostering a culture of creativity and resilience. Though he is no longer with us, his impact reverberates through the achievements of the HTL Biosolutions and the countless lives touched by his innovations and expertise. Dr. Li's memory remains a guiding force, steering HTL Biosolutions toward a future that honors and expands upon his visionary contributions.

Explore a selection of publications by Dr. Li below:

1. Arakawa, T., Ejima, D., Li, T. and Philo, J.S.  The critical role of mobile phase composition in size exclusion chromatography of protein pharmaceuticals.  J. Pharma. Sci., in press, 2009.


2. Li, C.H. and Li, T.  Application of Vibrational Spectroscopy to the Structural Analysis of Human Monoclonal Antibody Aggregates during Formulation Development.  Curr. Pharma. Biotech. 10, 391-399, 2009. 


3. Chelius, D., Jing, K., Lueras, A.M.K., Rehder, D., Dillon, T.M., Vizel, A., Rajan, S. R., Li, T., Treuheit, M. and Bondaernko, P.V.  Formation of Pyroglutamic Acid from N-Terminal Glutamic Acid in Immunoglobulin g Antibodies.  Anal. Chem.  78(7); 2370-2376, 2006.   


4. Rajan, R.S., Li, T., Aras, M., Sloey, C.,Sutherland, W., Arai, H., Briddell, R., Kinstler, O., Lueras, A. M.K.,  Zhang, Y., Yeghnazar, H., Treuheit, M. and Brems, D.N.  Modulation of protein aggregation by polyethylene glycol conjugation: GCSF as a case study.  Prot. Sci.  15, 1063-1075, 2006.


5. Li, T.  Investigation of Protein-Protein Interactions by Isotope Edited Fourier Transformed Infrared Spectroscopy.  Spectroscopy, 18, 397-406, 2004.


6. French, D., Arakawa, T. and Li, T.  FTIR Spectroscopic Investigation of Protein Conformation in Spray-Dried Protein-Trehalose Powders.  Biopolymers, 73(4 ), 524-531, 2004.  


7. Kerwin, B.A., Chang, B.S., Gegg, C.V., Gonelli, M., Li, T. and Strambini, G.  Interactions between PEG and type I soluble tumor necrosis factor receptor: Modulation by pH and by PEGylation at the N terminus. Protein Science. 11(7), 1825-1833, 2002.


8. Li, T., Talvenheimo, J., Zeni, L., Rosenfield, R., Stearns, G. and Arakawa, T.  Changes in Protein Conformation and Dynamics upon BDNF/trkB Complex Formation: An Investigation by Isotope-Edited FTIR Spectroscopy.  Biopolymers, 67(1), 10-19, 2002.


9. Li, T., Yamane, H., Arakawa, T., Narhi, L.O. and Philo, J.  The Effect of the Intermolecular Disulfide Bond on the Conformation and Stability of Glial Cell Line-Derived Neurotrophic Factor.  Prot. Eng., 15(1), 59-64, 2002.


10. Li, T., Narhi, L.O., Wen, J., Philo, J.S., Sitney, K., Inoue, J.-I., Yamamoto, T. and Arakawa, T.  Interactions between NFkB and Its Inhibitor ikB:  Biophysical Characterization of an NFkB/ikB Complex.  J. Prot. Chem.  17(8), 757-763, 1998.


11. Li, T., Horan, T., Osslund, T., Stearns, G. and Arakawa, T.  Conformational Changes in G-CSF/Receptor Complex As Investigated by Isotope-Edited FT-IR Spectroscopy. Biochemistry, 36, 8849-8857, 1997.


12. Eich, R.F., Li, T., Lemon, D.D., Doherty, D.H., Curry, S.R., Aitken, J.F., Mathews, A.J., Johnson,  K.A., Smith, R.D., Phillips, G.N., Jr. and Olson, J.S.  Mechanism of NO-Induced Oxidation of Myoglobin and Hemoglobin.  Biochemistry 35, 6976-6983, 1996.


13. Quillin, M., Li, T., Olson, J.S., Phillips, G.N., Jr., Dou, Y., Ikeda-Saito, M., Rega_n, R., Carlson, M., Gibson, Q.H., Li, H. and Elber, R.  Structural and Functional Effects of Apolar Mutations of the Distal Valine in Myoglobin.  J. Mol. Biol., 245, 416-436 (1995).


14. Li, T., Quillin, M., Phillips, G.N. and Olson, J.S., Structural Determinant of CO Stretching Vibrations in Myoglobins, Biochemistry, 33, 1433-1446 (1994).


15. Li, T., Johnson, J.E. and Thomas, G.J., Jr., Raman Dynamic Probe of Hydrogen Exchange in Bean Pod Mottle Virus:  Base-Specific Retardation of Exchange in Packaged ssRNA. Biophysical J., 65, 1963-1972 (1994).


16. Li, T., Bamford, J.K.H., Bamford, D.H. and Thomas, G.J., Jr., Structural Studies of the Eveloped dsRNA Bacteriophage ø6 of Pseudomonas syringae by Raman Spectroscopy.  I.  The Virion and Its Membrane, J. Mol. Biol.,  230, 461-472 (1993). 


17. Bamford, J.K.H., Bamford, D.H., Li, T. and Thomas, G.J., Jr., Structural Studies of the Eveloped dsRNA Bacteriophage ø6 of Pseudomonas syringae by Raman Spectroscopy.  II.  Nucleocapsid Structure and Thermostability of Virion, Nucleopsid and Polymerase Complex, J. Mol. Biol., 230, 473-482 (1993).


18. Li, T., Chen, Z., Johnson, J.E. and Thomas, G.J., Jr., Conformations, Interactions, and Thermostabilities of RNA and Proteins in Bean Pod Mottle Virus: Investigation of Solution and Crystal Structures by Laser Raman Spectroscopy, Biochemistry, 31, 6673-6682 (1992).


19. Li, T., Chen, Z., Johnson, J.E. and Thomas, G.J., Jr., Structural Studies of Bean Pod Mottle Virus, Capsid and RNA in Solution and Crystal States by Laser Raman Spectroscopy, Biochemistry, 29, 5018-5026 (1990).

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