top of page

cIEF

(Capillary Isoelectric Focusing)

Elevate your biomolecule analysis with cIEF. Uncover the charge variants and pI values that matter, offering precise insights for biopharmaceutical development and beyond. Explore the power of cIEF with us today.

About cIEF (Capillary Isoelectric Focusing)

 

Welcome to the world of Capillary Isoelectric Focusing (cIEF), where precision meets potential, and biomolecules reveal their hidden secrets. At HTL Biosolutions Inc, we specialize in the science of cIEF, a technique that empowers biotechnology by offering unparalleled insights into charge variants and isoelectric points (pI). Here's a closer look at what cIEF entails and its vital role in the realm of biotechnology:

cIEF builds upon the foundational principles of capillary gel electrophoresis. Electrophoresis is a separation technique that leverages the migration of charged molecules in response to an electric field, directing them toward an electrode of opposite charge. Traditionally, this method is performed within polyacrylamide gels. In the context of cIEF here's how it works:

  1. Sample Loading: A complex mixture of proteins or biomolecules is carefully loaded into a narrow capillary.

  2. pH Gradient Establishment: A pH gradient is meticulously created within the capillary using ampholyte mixtures. This gradient becomes the stage for our biomolecular performance.

  3. Isoelectric Focusing: An electric field is applied, driving the molecules through a pH gradient established within the capillary. As they migrate, biomolecules halt at their individual pI values, where it has a net charge of zero.

  4. High-Resolution Detection: At the precise moment each molecule reaches its pI, a sophisticated detector records the data. This information provides insights into charge variants and pI values.

Applications of cIEF (Capillary Isoelectric Focusing)

  1. Biopharmaceutical Quality Assurance: Ensuring the safety and efficacy of biopharmaceutical products by detecting charge variants, a crucial aspect of regulatory compliance.

  2. pI Determination: Accurate determination of a molecule's pI, which informs downstream processes and product development.

  3. Bioprocess Optimization: Enhancing bioprocessing efficiency by monitoring charge variants during production.

  4. Protein Characterization: In-depth analysis of post-translational modifications, structural variants, and charge distributions.

At HTL Biosolutions Inc, we harness the potential of cIEF to empower your biotechnological journey. Our state-of-the-art technology and expert team ensure that your research and development efforts are underpinned by the highest standards of quality and precision.

Contact us today to embark on a journey of deeper understanding through the power of cIEF.

bottom of page