About CD (Circular Dichroism) Spectroscopy

 

Welcome to the forefront of molecular analysis at HTL Biosolutions. Our Circular Dichroism (CD) Spectroscopy services are meticulously designed to address the unique needs of the biotechnology sector, offering transformative insights.

 

Circular Dichroism (CD) Spectroscopy is a powerful analytical technique used in chemistry, biochemistry, and structural biology to study the chiral properties of small molecules, and higher order structure of biomolecules like proteins, nucleic acids, and peptides. CD spectroscopy is also a powerful tool for comparability and similarity analysis of biomolecules.

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Here's how CD spectroscopy works:

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1. Polarized Light: CD spectroscopy involves shining plane-polarized light through a sample containing chiral properties. Chiral molecules or components interact differently with left-circularly polarized light (L-CPL) and right-circularly polarized light (R-CPL).

2. Chiral Interactions: Chiral molecules or components absorb L-CPL and R-CPL differently due to their asymmetrical structures. This differential absorption results in what is known as circular dichroism, which is the difference in the absorption of the two circularly polarized light components.

3. CD Spectrum: The CD spectrometer measures this difference in absorbance as a function of wavelength, generating a CD spectrum. The CD spectrum provides information about the secondary structure, conformation, and chiral properties of the molecules in the sample.

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Applications of CD (Circular Dichroism) Spectroscopy

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UV Circular Dichroism (CD) Spectroscopy stands as a potent analytical technique that empowers us to unravel the intricate structures of biomolecules. This technique is the key to unlocking a multitude of applications in biotechnology, including:

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1. Higher Order Structure Characterization: Gain a profound understanding of biomolecule higher-order structures (secondary and tertiary structure) and assess their stability, essential for ensuring product quality.

2. Comparability/Similarity Analysis: Confirm the comparability or similarity of biopharmaceutical products, including biosimilars, by analyzing their CD spectra to ensure consistent structural and functional attributes.

3. Conformational Changes Under Stress or Formulation Conditions: Monitor conformational changes in biomolecules under various conditions, providing insights into stability

4. Protein Folding and Unfolding: Investigate the kinetics and thermodynamics of protein folding and unfolding

5. Protein-Ligand Interactions: Study interactions between proteins and ligands, elucidating binding mechanisms that are vital for drug discovery and development.

6. Chirality of Small Molecule APIs: Determine the chirality (stereochemistry) of small molecule Active Pharmaceutical Ingredients (APIs), ensuring the correct form is used in drug formulations.

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At HTL Biosolutions, we are dedicated to bringing the power of CD Spectroscopy to your biotechnology endeavors. Our team of experts, armed with state-of-the-art technology and extensive knowledge, ensures your success in every project. We are not just service providers; we are your partners in molecular analysis.

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Contact us today to harness the capabilities of CD Spectroscopy and enhance your biotechnology projects.

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