HPLC

At HTL Biosolutions, Inc., we provide analytical services on a full range of HPLC analysis of proteins, including size-exclusion (SEC), ion-exchange (IEX), hydrophobic interaction (HIC) and reversed-phase (RP) HPLC chromatography. We also provide peptide-mapping service according to customer's specific request. 

 

Key Benefits

Analysis of protein aggregates 

Analysis of protein degradants such as clip, cyclization and fragmentation

Analysis of chemical modification of proteins such as oxidation, deamindation 

 

Capabilities 

Size-exclusion chromatography (SEC-HPLC)

This technique is often used to provide quantitative analysis of protein aggregates and clips. 

 

Ion-exchange chromatography (IEX)

Anion-exchange to characterize protein charge isoforms, and deamindated species. Cation-exchange to characterize protein charge isoforms, and deamindated species. 

 

Reversed-phase chromatography (RP-HPLC)

Quantitative analysis of protein degradants such as oxidation, deamindation and clips. 

 

Hydrophobic interaction chromatography (HIC)

Analysis of protein variants under non-denaturing condition. 

 

Peptide Mapping 

Determination of degradations occurring at specific amino acid positions.

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